The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as , belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including , , , , and was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify , , , , and gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus . The phylogenetic analysis results indicated that T011 was accepted at subclade and significantly formed the monophyletic group with referent (Telemorph of ) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the . In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284333 | PMC |
| http://dx.doi.org/10.1515/biol-2021-0074 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Studies on trematodes and acanthocephalans from freshwater fishes of Hubei Province, central China, with the erection of a new genus Quadrihexaspiron gen. n. (Acanthocephala: Neoechinorhynchidae).
Folia Parasitol (Praha)
January 2025
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei Province, People's Republic of China *Address for correspondence: Frantisek Moravec, Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic. E-mail: ORCID: 0000-0003-1086-1181.
The present paper comprises a systematic survey of trematodes and acanthocephalans based on helminthological examinations of 64 specimens of 14 species of freshwater fishes, belonging to six families of four fish orders, mostly from localities in Hubei Province, central China, collected in the autumn of 2002. A total of 15 trematode species (in 12 families) and 5 acanthocephalan species (in four families) was recorded. Almost all parasites are briefly described and illustrated and problems concerning their morphology, taxonomy, hosts and geographical distribution are discussed.
View Article and Find Full Text PDFChromosome-scale telomere to telomere genome assembly of common crystalwort (Riccia sorocarpa Bisch.).
Sci Data
January 2025
Department of Botany and Evolutionary Ecology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, Olsztyn, 10-719, Poland.
Riccia sorocarpa Bisch., commonly known as common crystalwort, is a plant belonging to the Marchantiales order with a cosmopolitan distribution among a wide range of habitats: fields, gardens, waste ground, on paths, cliff tops, and thin soil over rocks or by water bodies. However, research into the genetic aspects of this species is limited.
View Article and Find Full Text PDFHILIC-MS/MS Multi-targeted Method for Metabolomics Applications.
Methods Mol Biol
January 2025
Biomic Auth, Bioanalysis and Omics Laboratory, Center for Interdisciplinary Research and Innovation, Aristotle University, Thessaloniki, Greece.
Metabolomics aims at identification and quantitation of key end point metabolites, basically polar, in order to study changes in biochemical activities in response to pathophysiological stimuli or genetic modifications. Targeted profiling assays enjoying a growing popularity over the last years with LC-MS/MS as a powerful tool for development of such (semi-)quantitative methods for a large number of metabolites. Here we describe a method for absolute quantitation of ca.
View Article and Find Full Text PDFTwo rodent suborders have evolved missing amino acids in the lipid-binding region of apolipoprotein E.
Lipids
January 2025
Molecular Biology Institute, University of California, Los Angeles, California, USA.
The order Rodentia comprises nearly 45% of all extant taxa, currently organized into 31 living families, some 450 genera, and roughly 2010 species (Kelt & Patton, 2020). Considering that rodents began evolving at least 66 million years ago, it is not surprising that they have diversified into five distinct suborders. With the advent of molecular biology, this difference can often be seen at the molecular level as well.
View Article and Find Full Text PDFDiscovery and mechanistic exploration of promiscuous xylosyltransferase based on protein engineering.
Int J Biol Macromol
January 2025
National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Beijing 100700, China.
Glycosylation is an effective means to alter the structure and properties of plant compounds, influencing the pharmacological activity of natural products (NPs) to obtain highly active NPs. In nature, glucosides are the most widely distributed, while other glycosides such as xylosides are less common and present in lower quantities. This is due to the scarcity of xylosyltransferases with substrate promiscuity in nature, and the modification of their catalytic function is also quite challenging.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!