Thermophilin 110 inhibits growth and biofilm formation of .

Dental caries continues to occur in both children and adults worldwide resulting in significant economic burden, and consumers have expressed interest in natural products that can prevent these recurrent infections. In this study, B59671, which produces thermophilin 110, was shown to inhibit the growth of UA159. A thermophilin concentration ≥ 80 AU ml prevented the growth of UA159 in batch culture, while ≥ 160 AU ml was required to prevent biofilm growth. Co-culturing B59671 and UA159 also resulted in impaired biofilm growth. Thermophillin 110 was also shown inhibit additional strains and commensal oral streptococci at higher concentrations (640-1280 AU ml). These results suggest that thermophilin 110 could be used as a natural antimicrobial in oral care products and support the need for additional studies to assess the probiotic potential of B59671.