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Transcriptional Profile of Cytokines, Regulatory Mediators and TLR in Mesenchymal Stromal Cells after Inflammatory Signaling and Cell-Passaging. | LitMetric

AI Article Synopsis

  • Adult human adipose tissue contains mesenchymal stromal cells (MSCs) that are important for tissue repair, and their response to inflammatory and proliferative signals is crucial to study.* -
  • The research characterized the transcriptional profiles of cytokines and Toll-like receptors (TLRs), revealing specific patterns in MSC responses to inflammation, such as increased expression of IL-6, IL-8, and TNFα.* -
  • The findings indicate that inflammation can alter the sensitivity and responsiveness of AT-MSCs, providing insights that could lead to new therapeutic strategies for tissue repair.*

Article Abstract

Adult human subcutaneous adipose tissue (AT) harbors a rich population of mesenchymal stromal cells (MSCs) that are of interest for tissue repair. For this purpose, it is of utmost importance to determine the response of AT-MSCs to proliferative and inflammatory signals within the damaged tissue. We have characterized the transcriptional profile of cytokines, regulatory mediators and Toll-like receptors (TLR) relevant to the response of MSCs. AT-MSCs constitutively present a distinct profile for each gene and differentially responded to inflammation and cell-passaging. Inflammation leads to an upregulation of IL-6, IL-8, IL-1β, TNFα and CCL5 cytokine expression. Inflammation and cell-passaging increased the expression of HGF, IDO1, PTGS1, PTGS2 and TGFβ. The expression of the TLR pattern was differentially modulated with TLR 1, 2, 3, 4, 9 and 10 being increased, whereas TLR 5 and 6 downregulated. Functional enrichment analysis demonstrated a complex interplay between cytokines, TLR and regulatory mediators central for tissue repair. This profiling highlights that following a combination of inflammatory and proliferative signals, the sensitivity and responsive capacity of AT-MSCs may be significantly modified. Understanding these transcriptional changes may help the development of novel therapeutic approaches.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306573PMC
http://dx.doi.org/10.3390/ijms22147309DOI Listing

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