The difference in lethality to cycloheximide between actively dividing and non-dividing cells was exploited to enhance detection of auxotrophic and UV-sensitive mutants in the fungi Schizophyllum commune and Saccharomyces cerevisiae.
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A dominant negative 14-3-3 mutant in Schizosaccharomyces pombe distinguishes the binding proteins involved in sexual differentiation and check point.
PLoS One
October 2023
Faculty of Life and Environmental Sciences, Department of Life Sciences, Shimane University, Matsue, Shimane, Japan.
The homothallic fission yeast Schizosaccharomyces pombe undergoes sexual differentiation when starved, but sam (skips the requirement of starvation for mating) mutants such as those carrying mutations in adenylate cyclase (cyr1) or protein kinase A (pka1) mate without starvation. Here, we identified sam3, a dominant negative allele of rad24, encoding one of two 14-3-3 proteins. Genetic mapping and whole-genome sequencing showed that the sam3 mutation comprises a change in nucleotide at position 959 from guanine to adenine, which switches the amino acid at position 185 from glutamic acid to lysine (E185K).
View Article and Find Full Text PDFSex-linked gene traffic underlies the acquisition of sexually dimorphic UV color vision in butterflies.
Proc Natl Acad Sci U S A
August 2023
Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697.
The acquisition of novel sexually dimorphic traits poses an evolutionary puzzle: How do new traits arise and become sex-limited? Recently acquired color vision, sexually dimorphic in animals like primates and butterflies, presents a compelling model for understanding how traits become sex-biased. For example, some butterflies uniquely possess UV (ultraviolet) color vision, which correlates with the expression of two differentially tuned UV-sensitive rhodopsins, UVRh1 and UVRh2. To discover how such traits become sexually dimorphic, we studied , which exhibits female-specific UVRh1 expression.
View Article and Find Full Text PDFMUS81 is required for atypical recombination intermediate resolution but not crossover designation in rice.
New Phytol
March 2023
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, 225009, Yangzhou, China.
The endonuclease methyl methanesulfonate and UV-sensitive protein 81 (MUS81) has been reported to participate in DNA repair during mitosis and meiosis. However, the exact meiotic function of MUS81 in rice remains unclear. Here, we use a combination of physiological, cytological, and genetic approaches to provide evidence that MUS81 functions in atypical recombination intermediate resolution rather than crossover designation in rice.
View Article and Find Full Text PDFDNA interstrand crosslink repair by XPF-ERCC1 homologue confers ultraviolet resistance in Neurospora crassa.
Fungal Genet Biol
January 2023
Laboratory of Genetics, Division of Life Science, Graduate School of Science & Engineering, Saitama University, Shimo-Ohkubo 255, Sakura Ward, Saitama City, Saitama 338-8570, Japan. Electronic address:
Ultraviolet (UV) light is a mutagen that causes DNA damage. Some UV-sensitive Neurospora crassa strains have been reported to exhibit a partial photoreactivation defect (PPD) phenotype, and the possible cause of this has been unknown for more than half a century. In this study, in the process of elucidating the possible causes of a PPD phenotype, we discovered that the XPF homologue MUS-38 is involved in repairing the UV-induced DNA interstrand crosslink (ICL) in N.
View Article and Find Full Text PDFOpsin knockdown specifically slows phototransduction in broadband and UV-sensitive photoreceptors in Periplaneta americana.
J Comp Physiol A Neuroethol Sens Neural Behav Physiol
November 2022
Department of Physiology and Biophysics, Dalhousie University, P.O. BOX 15000, Halifax, NS, B3H 4R2, Canada.
Photoreceptors with different spectral sensitivities serve different physiological and behavioral roles. We hypothesized that such functional evolutionary optimization could also include differences in phototransduction dynamics. We recorded elementary responses to light, quantum bumps (QBs), of broadband green-sensitive and ultraviolet (UV)-sensitive photoreceptors in the cockroach, Periplaneta americana, compound eyes using intracellular recordings.
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