Evaluation of Different Extraction Methods for the Analysis of Per- and Polyfluoroalkyl Substances in Dried Blood Spots from the Florida Manatee (Trichechus manatus).

Per- and polyfluoroalkyl substances (PFAS) are environmental contaminants linked to adverse health effects in humans and wildlife. Marine mammals, particularly manatees, have shown potential as sentinels for evaluating the presence and effects of anthropogenic chemicals. However, traditional blood collection techniques with marine mammals can be challenging, highlighting the need for improved strategies. In the present study, four different methods for the extraction of PFAS from dried blood spots were evaluated. Environ Toxicol Chem 2021;40:2726-2732. © 2021 SETAC.