Affinity chromatography can be repurposed to provide useful information about the specific partner protein(s) to which a protein of interest may bind as well as the relative binding affinity of that partner protein for the protein of interest. Here, we provide a protocol for an Ni-NTA affinity chromatography assay that may be utilized to uncover insightful information about the nature of protein-protein interactions during iron-sulfur (Fe-S) cluster biogenesis reactions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8969215 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-1605-5_7 | DOI Listing |
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