Use of polylactic acid microvessel to obtain microplantlets of through indirect organogenesis.

Microplants of were produced through indirect organogenesis, and the interaction of plant growth regulators (PGRs) (TDZ-thidiazuron and NAA-α-naphthalene acetic acid), juvenile tissues (cotyledon and hypocotyl) and different types of polylactic acid (PLA) microvessels on plant production were evaluated. Cotyledon-derived callus induction increased by 30-60% in all tested combinations of TDZ and NAA concentrations compared the absence of PGRs. Hypocotyl-derived callus induction was improved in most tested combinations of TDZ and NAA concentrations. Moreover, 100% callus induction from both tissues was achieved with TDZ (1, 2 and 3 mg L) + NAA (0 mg L). Bud induction from cotyledon tissues was improved with TDZ (1 and 3 mg L) + NAA (0 mg L) and from hypocotyl with TDZ (1 and 2 mg L) + NAA (0 mg L). Shoot elongation from cotyledon tissues was not improved from any combination of PGRs, whereas TDZ (1 mg L) + NAA (0 mg L), TDZ (1 mg L) + NAA (4 mg L), TDZ (2 mg L) + NAA (4 mg L) and TDZ (3 mg L) + NAA (2 mg L) improved shoot elongation from hypocotyl tissues. Adventitious rooting and acclimatization of microcuttings ranged from 40 to 70% in three of the tested microvessels. The acclimatized microcuttings had low genetic variability. Successful production of microplants was achieved in this study using hypocotyl tissue and cultivated a culture medium supplemented with TDZ and NAA, using PLA-based microvessels.