is a traditional polyherbal formula used in Indian Ayurvedic and Chinese Tibetan medicine. A wide range of biological activities have been attributed to , but the impact of various extraction methods on efficacy has not been determined. The study aimed to evaluate extracts obtained by various methods for their hepatoprotective effects and molecular mechanisms in a mouse model of carbon tetrachloride (CCl)-induced liver injury. HPLC fingerprinting was used to characterize the chemical characteristics of extracts obtained by (a) 0.5 h ultrasonication, (b) 2 h reflux, and (c) 4 h reflux. Hepatoprotective efficacy was evaluated in a mouse model of CCl-induced liver damage. Serum levels of alanine transaminase (ALT) and aspartate aminotransferase (AST) were measured, as well as the liver antioxidant and inflammatory markers malondialdehyde superoxide dismutase glutathione peroxidase (GSH-Px), TNF-α, and IL-6. Gene and protein expression of Nrf-2 signaling components Nrf-2, heme oxygenase (HO-1), and NADPH Quinone oxidoreductase (NQO-1) in liver tissue were evaluated by real-time PCR and western blotting. Chemical analysis showed a clear difference in content between extracts produced by ultrasonic and reflux methods. The pharmacological analysis showed that all three Triphala extracts reduced ALT, AST, MDA, TNF-α, and IL-6 levels and increased SOD and GSH-Px. Triphala extracts also induced transcript and protein expression of Nrf-2, HO-1, and NQO-1. Triphala extract prevents CCl-induced acute liver injury. The ultrasonic extract of Triphala was most effective, suggesting that hepatoprotection may be related to the larger tannins via activation of Nrf-2 signaling.
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http://dx.doi.org/10.3389/fphar.2021.664607 | DOI Listing |
Talanta
January 2025
School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China. Electronic address:
As a serine hydrolase synthesized by the liver, butyrylcholinesterase (BChE) is an important biomarker in the clinical diagnosis of liver diseases. To track BChE activity in drug-induced liver injury, we designed a deep-red BChE-activatable fluorescent probe (CYL-BChE) with hemi-cyanine structure by using a cyclopropyl carbonyl group as a specific recognition moiety. Its near-infrared absorption wavelength (665 nm) and emission wavelength (762 nm) provide excellent tissue penetration capabilities, making it suitable for biological imaging.
View Article and Find Full Text PDFEXCLI J
November 2024
Department of Life Sciences, Faculty of Science and Engineering, Manchester Metropolitan University, Manchester M1 5GD, UK.
Farnesol (FAR) is a sesquiterpene alcohol that exists in many fruits and vegetables and possesses promising anti-inflammatory and antioxidant activities. Cadmium (Cd) is an environmental pollutant known for its serious health effects. Liver injury associated with oxidative stress is a hazardous consequence of exposure to Cd.
View Article and Find Full Text PDFACG Case Rep J
January 2025
Stony Brook Medicine, Department of Medicine, Division of Gastroenterology and Hepatology, Stony Brook University Hospital, Stony Brook, NY.
Although herpes simplex virus, Epstein-Barr virus, and hemophagocytic lymphohistiocytosis are known causes of severe acute liver injury with or without liver failure, these diseases occur almost exclusively in immunocompromised and elderly patients. We report a case of an immunocompetent young man with no medical history who presented with a subacute cough and persistent fevers in the setting of a penile chancre. He was found to have severely elevated liver chemistries and was subsequently diagnosed with hemophagocytic lymphohistiocytosis because of disseminated herpes simplex virus type 1 and Epstein-Barr virus coinfection.
View Article and Find Full Text PDFInfect Drug Resist
January 2025
Department of Immunology and Rheumatology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, People's Republic of China.
Purpose: Sepsis-associated liver injury (SALI) leads to increased mortality in sepsis patients, yet no specialized tools exist for early risk assessment. This study aimed to develop and validate a risk prediction model for early identification of SALI before patients meet full diagnostic criteria.
Patients And Methods: This retrospective study analyzed 415 sepsis patients admitted to ICU from January 2019 to January 2022.
ACS Appl Mater Interfaces
January 2025
College of pharmacy, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
This study introduced a hydrogel dressing, termed SODex-gel, which was constructed by establishing Schiff base and hydrogen bonds with the precursors of oxidized dextran (ODex) and succinic dihydrazide (SD)-modified sodium alginate (SD--SA). Through comprehensive and studies, the adhesive properties, self-healing capabilities, hemostatic potential, and wound healing efficacy of the SODex-gel dressing were meticulously evaluated. The H NMR, FTIR, and TGA analyses confirmed the fabrication of the SODex-gel dressing and its constituent elements.
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