AI Article Synopsis
- Cyrtorhinus lividipennis, a predator of rice planthopper eggs, relies on the enzyme alanine aminotransferase (ClALT) for crucial physiological functions, with higher expression found in the fat body and midgut.
- Silencing ClALT through RNA interference significantly impaired its activity and expression, impacting amino acid levels in the hemolymph, and causing reductions in vital proteins and reproductive factors in females.
- Results indicate that ClALT plays a significant role in amino acid metabolism and overall reproductive success, affecting egg laying and ovarian development in C. lividipennis.
Article Abstract
In Asian rice systems, Cyrtorhinus lividipennis Reuter is an important predator that preys on rice planthopper eggs and young nymphs, as a primary food source. Alanine aminotransferase (ALT) acts in many physiological and biochemical processes in insects. We cloned the full-length complementary DNA of C. lividipennis ClALT. Expression analysis showed higher expression in the fat body and midgut compared to other tissues. It is expressed in all C. lividipennis developmental stages and at least four organs. Silencing of ClALT by RNA interference significantly decreased the ClALT enzyme activity and ClALT expression compared to dsGFP-treated controls at 2 days after emergence (DAE). Silencing of ClALT influenced free hemolymph amino acid compositions, resulting in a reduction of Aspartic acid (Asp) and Alanine (Ala) proportions, and increased Cysteine (Cys) and Valine (Val) proportions in females at 2 DAE. dsClALT treatments led to decreased soluble total protein concentrations in ovary and fat body, and to lower reduced vitellogenin (Vg) expression, body weight, and the numbers of laid eggs. The double-stranded RNA viruse treatments also led to prolonged preoviposition periods and hindered ovarian development. Western blot analysis indicated that silencing ClALT also led to reduced fat body Vg protein abundance at 2 DAE. These data support our hypothesis that ClALT influences amino acid metabolism and fecundity in C. lividipennis.
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| http://dx.doi.org/10.1002/arch.21836 | DOI Listing |
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