Description of sp. nov., isolated from deep sea sediment from the Mariana Trench, and emended description of the genus .

In this study, we reported a Gram-stain-negative, orange-coloured, rod-shaped, motile and faculatively anaerobic bacterium named strain PB63, which was isolated from the deep-sea sediment from the Mariana Trench. Growth of PB63 occurred at 10-35 °C (optimum, 28 °C), pH 5.0-8.0 (optimum, 5.0-6.0) and with 0-7 % (w/v) NaCl (optimum, 2-3 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that PB63 represented a member of the genus and was closely related to DSM 16222 (97.9 % sequence similarity). PB63 showed tolerance to a variety of heavy metals, including Co, Zn, Mn and Cu. The complete genome of PB63 was obtained, and many genes involved in heavy metal resistance were found. The genomic DNA G+C content of PB63 was 62.8 mol%. The predominant respiratory quinone of PB63 was ubiquinone-10 (Q-10). The polar lipids of PB63 contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, glycolipid, phosphatidylcholines and three unidentified lipids. The major fatty acids of PB63 included summed feature 8 (Cω7 or/and Cω6), C 2-OH, 11-methyl Cω7c, C, summed feature 3 (Cω7 and/or Cω6) and Cω6. The results of phylogenetic, physiological, biochemical and morphological analyses indicated that strain PB63 represents a novel species of the genus , and the name sp. nov. is proposed with the type species PB63 (=CCTCC AB 2019195=JCM 34178).