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Establishment of a Patient-Derived, Magnetic Levitation-Based, Three-Dimensional Spheroid Granuloma Model for Human Tuberculosis. | LitMetric

Establishment of a Patient-Derived, Magnetic Levitation-Based, Three-Dimensional Spheroid Granuloma Model for Human Tuberculosis.

mSphere

DST-NRF Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Councilgrid.415021.3 Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch Universitygrid.11956.3a, Cape Town, South Africa.

Published: August 2021

AI Article Synopsis

  • Tuberculous granulomas, formed in response to Mycobacterium tuberculosis (M. tuberculosis) infection, are complex structures influenced by the immune response, cellular interactions, and pathogen-induced changes in host cell behavior.
  • A new three-dimensional human lung spheroid granuloma model mimics early stages of TB granulomas and allows researchers to examine immune cell behaviors, host responses, and treatment effects more accurately than traditional animal models.
  • Understanding granulomas is crucial for shedding light on M. tuberculosis infection control, yet studying them in humans is challenging, which makes this innovative model significant for advancing TB research.

Article Abstract

Tuberculous granulomas that develop in response to Mycobacterium tuberculosis (M. tuberculosis) infection are highly dynamic entities shaped by the host immune response and disease kinetics. Within this microenvironment, immune cell recruitment, polarization, and activation are driven not only by coexisting cell types and multicellular interactions but also by M. tuberculosis-mediated changes involving metabolic heterogeneity, epigenetic reprogramming, and rewiring of the transcriptional landscape of host cells. There is an increased appreciation of the complexity, versatility, and heterogeneity of the cellular compartment that constitutes the tuberculosis (TB) granuloma and the difficulty in translating findings from animal models to human disease. Here, we describe a novel biomimetic three-dimensional (3D) human lung spheroid granuloma model, resembling early "innate" and "adaptive" stages of the TB granuloma spectrum, and present results of histological architecture, host transcriptional characterization, mycobacteriological features, cytokine profiles, and spatial distribution of key immune cells. A range of manipulations of immune cell populations in these spheroid granulomas will allow the study of host/pathogen pathways involved in the outcome of infection, as well as pharmacological interventions. TB is a highly infectious disease, with granulomas as its hallmark. Granulomas play an important role in the control of M. tuberculosis infection and as such are crucial indicators for our understanding of host resistance to TB. Correlates of risk and protection to M. tuberculosis are still elusive, and the granuloma provides the perfect environment in which to study the immune response to infection and broaden our understanding thereof; however, human granulomas are difficult to obtain, and animal models are costly and do not always faithfully mimic human immunity. In fact, most TB research is conducted on immortalized or primary immune cells and cultured in two dimensions on flat, rigid plastic, which does not reflect characteristics. We have therefore conceived a 3D, human spheroid granuloma model which allows researchers to study features of granuloma-forming diseases in a 3D structural environment resembling granuloma architecture and cellular orientation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386456PMC
http://dx.doi.org/10.1128/mSphere.00552-21DOI Listing

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