Nitrate reductase (NR) reduces the major plant nitrogen source, NO-, into NO-. NR activity can be measured by its final product, nitrite through its absorbance under optimized condition. Here, we present a detailed protocol for measuring relative enzyme activity of NR from crude extracts. This protocol offers simple procedure and data analysis to compare NR activity of multiple samples.
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| http://dx.doi.org/10.21769/BioProtoc.2785 | DOI Listing |
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