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Homeostasis between the cytoplasmic plant hormone salicylic acid (SA) and its' inactive, vacuolar storage forms, SA-2-β-D-glucoside (SAG) and SA-β-D-Glucose Ester (SGE), regulates the fine-tuning of defense responses to biotrophic pathogens in . This protocol describes a simplified, optimized procedure to extract and quantify free SA and total hydrolyzable SA in plant tissues using a classical HPLC-based method.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275313 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2844 | DOI Listing |
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