Fruit-derived polyphenolic compounds have been shown to exert anticancer effects epigenetic mechanisms. In this study, we investigated the effect of blackberry extract on the expression of DNMTs (, , and ) and HDACs ( and ) and its influence on the cellular differentiation and promoter DNA methylation of tumor-related genes using a panel of six human CRC cell lines. Treatment with IC and IC concentrations of blackberry extract for 72 h significantly reduced and transcript levels in HCT116, SW480, HT29/219, SW742, and LS180 cells in a dose-dependent manner. Blackberry also induced promoter DNA demethylation of and genes in four tested CRC cell lines. Berry treatment, however, upregulated genes in SW480, SW742, and HT29/219 cell lines. A dose-dependent and cell-type-specific reduction of , , and expressions were observed in CRC-treated cells. Treatment with berry extract induced the expression of gene in HCT116 and HT29/219 cells and increased the expression of two colonic epithelial cell differentiation markers, carcinoembryonic antigen (CEA) and alkaline phosphatase in LS180 cells in a time-dependent manner. This study is the first to report the epigenetic effects of blackberry in cancer cells.
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