Purpose: The present study aimed to research the cytotoxic effects of Colchicum baytopiorum extract on normal and cancerous cells and reveal the cell death mechanisms in cancerous cells triggered by this effect.
Methods: Within this framework, the cells' index values obtained with an xCELLigence Real Time Cell Analysis DP device, selectivity index (SI), apoptotic index (AI) based on a DAPI application and time-related activities of caspase 3,7 and 9 with a spectrofluorometer were inspected. The expressions of apoptosis/autophagy/entosis/necroptosis/anoikis-related genes were researched with qRT-PCR.
Results: It was determined that C. baytopiorum extract had displayed a high selectivity [(SI)=4], increased AI (p<0.01) and activation of caspases 3,7 and 9 (p<0.05). It was observed that the mRNA expressions of Atg12, Atg16, Atg5, Atg7, bad, bak, bax, bcl-xL, Beclin1, caspase3, FLIP, Puma, LC3, mcl-1, Bit1, Rho, RIP1, ROCK and TRAF2 genes in C-4 I cells to which the plant extract was applied had increased significantly in comparison with the control group (FC≥1.5). A lowering was detected in the mRNA levels of IAP, SRC kinase and TNF.
Conclusion: Consequently, it was revealed that the plant extract used had increased the gene expressions in the autophagic cell death pathway in C-4 I cells along with apoptosis and thus, it could be a promising candidate for cervix carcinoma treatment.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Phytochemical, Cytoprotective Profiling, and Anti-Inflammatory Potential of in Rheumatoid Arthritis: An Experimental and Simulation Study.
Nutrients
November 2024
Institute of Biomedical and Genetic Engineering (IBGE), Sector G-9/4, Islamabad 44000, Pakistan.
Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by severe pain, inflammation, and joint deformity. Currently, it affects 1% of the population, with a projection to exceed 23 million cases by 2030. Despite significant advancements, non-steroidal anti-inflammatory drugs (NSAIDs), the first line of treatment, are associated with a range of adverse effects.
View Article and Find Full Text PDFColchicine reduces neointima formation and VSMC phenotype transition by modulating SRF-MYOCD activation and autophagy.
Acta Pharmacol Sin
December 2024
Anhui Provincial International Science and Technology Cooperation Base for Major Metabolic Diseases and Nutritional Interventions, Key Laboratory of Metabolism and Regulation for Major Diseases of Anhui Higher Education Institutes, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230601, China.
Vascular smooth muscle cell (VSMC) phenotype transformation significantly contributes to vascular intimal hyperplasia. However, effective preventive and therapeutic measures are lacking. Colchicine, a binary alkaloid derived from Colchicum autumnale, is traditionally used for treating inflammatory diseases.
View Article and Find Full Text PDFUnderstanding the effects of weather parameters on the population dynamics of an endangered geophyte supports monitoring efficiency.
Sci Rep
October 2024
'Lendület' Seed Ecology Research Group, Institute of Ecology and Botany, HUN-REN Centre for Ecological Research, Vácrátót, Hungary.
Article Synopsis
- Geophytes, like Colchicum bulbocodium, are often overlooked in conservation efforts, despite their significance in early spring ecosystems and complex life cycles.
- Weather factors such as temperature, precipitation, and light greatly influence their life cycles, but these effects can vary widely, making understanding them crucial for effective conservation strategies.
- The study found that rising temperatures and insufficient cold periods negatively impact all life stages of C. bulbocodium, revealing that relying on a single year's flowering count can significantly underestimate population size, emphasizing the need for multi-year monitoring after colder, wetter conditions.
The Effects of Colchicum Dispert and Bone Marrow-Derived Mesenchymal Stem Cell Therapy on Skeletal Muscle Injury in a Rat Aortic Ischemia-Reperfusion Model.
J Cardiovasc Dev Dis
August 2024
Department of Biochemistry, Medical Faculty, Selçuk University, Konya 42250, Turkey.
Background: Abdominal aortic aneurysms and peripheral artery disease pose significant health risks, ranking third after heart attacks and cerebral strokes. Surgical interventions often involve temporary aortic clamping, leading to ischemia-reperfusion injury and tissue damage. Colchicine and mesenchymal stem cells have shown promise, individually, in mitigating ischemia-reperfusion injury, but their combined effects remain understudied.
View Article and Find Full Text PDFMicropropagation and cryopreservation of the rare endemic Colchicum figlalii germplasm.
Cryo Letters
May 2024
Çukurova University, Faculty of Agriculture, Department of Horticulture, 01250 Sarıçam-Adana, Turkey.
Background: The natural population of Colchicum figlalii (Varol) Parolly and Eren grows in a narrow area of serpentine rock clearings at an altitude of 1900-2100 m in Southwestern Anatolia (Sandras Mountain, Mugla, Turkey). The species is regarded as endangered according to the IUCN Red List Categories.
Objective: To develop an optimum procedure for in vitro propagation and cryopreservation of germplasm of this rare endemic.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!