Antibiofilm action of glycolic extract over and absence of toxicity in .

Objectives: This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic extract of "" over multidrug-resistant strains of as alternative therapy to be investigated.

Methods: A bacterial inoculum of each bacterial strain (4a, 5a, 9a, 12a, ATCC 19606) of was prepared and adjusted by the spectrophotometer. The microdilution broth method was performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). glycolic extracts were obtained of the tree stalk and leaves. The biofilm viability was tested by MTT assay after 5 min exposure. The toxicity of the extracts was tested by invertebrate model The data were analyzed by ANOVA, Tukey test and log-rank method (=0.05).

Results: The extract showed an inhibitory and bactericidal action over all the tested strains with the lowest MIC value observed for the reference strain (3.12 mg/mL). The extract did not demonstrate toxicity in any of the tested concentrations (12.5, 25 and 50 mg/mL) in mellonella larvae, with a survival percentage above 80% after 168 h.

Conclusions: The glycolic extract of has microbicidal and antibiofilm activity on multidrug-resistant clinical strains of and showed low toxicity for the invertebrate model