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- * Results showed that the allicin and 5-FU combination significantly increased cancer cell apoptosis and reduced levels of specific proteins associated with drug resistance.
- * The findings suggest that using allicin alongside 5-FU may be an effective strategy to combat multidrug resistance in gastric cancer treatment.
Article Abstract
Background & Objective: 5-fluorouracil (5-FU) is approved for the treatment of gastric carcinoma (GC), but chemo-resistance limits the application of it for GC. Thus, the combination of 5-FU with adjuvants such as allicin may overcome multidrug resistance (MDR).
Methods: The anticancer effects of allicin, 5-FU, and allicin/5-FU on the 5-FU resistant MKN-45 cells were evaluated by MTT assay and DAPi staining. The expression of the P-glycoprotein (P-gp) and CD44 protein were determined using immunocytochemistry. We also quantified mRNA expression levels of (), and in the GC cells.
Results: Here, we found that the combination of allicin with 5-FU significantly increased apoptosis compared to 5-FU alone (<0.05). We showed that , and mRNA expression levels were down-regulated in the allicin- and allicin/5-FU-treated cells. Indeed, the combination of allicin and 5-FU significantly decreased the expression of the P-gp and CD44 proteins (<0.05).
Conclusion: Our findings indicate that the combination of allicin with 5-FU could reverse multidrug resistance in the GC cells by reducing the expression of , , P-gp, and CD44 levels.
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| http://dx.doi.org/10.1055/a-1525-1499 | DOI Listing |
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