AI Article Synopsis
- - Glycerol phosphate (GroP)-based teichoic acids (TAs) are important cell-wall components found in enterococcus and staphylococcus bacteria, and their role in immune responses has been studied.
- - This research is the first to investigate how a monoclonal antibody interacts with synthetic TAs at a molecular level, using various techniques like microarrays and spectroscopy.
- - The study reveals that the structure of GroP residues, particularly their quantity and orientation, is key to the antibody's binding, with sugar attachments playing a significant role in presenting the structure to the antibody.
Article Abstract
Glycerol phosphate (GroP)-based teichoic acids (TAs) are antigenic cell-wall components found in both enterococcus and staphylococcus species. Their immunogenicity has been explored using both native and synthetic structures, but no details have yet been reported on the structural basis of their interaction with antibodies. This work represents the first case study in which a monoclonal antibody, generated against a synthetic TA, was developed and employed for molecular-level binding analysis using TA microarrays, ELISA, SPR-analyses, and STD-NMR spectroscopy. Our findings show that the number and the chirality of the GroP residues are crucial for interaction and that the sugar appendage contributes to the presentation of the backbone to the binding site of the antibody.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389533 | PMC |
| http://dx.doi.org/10.1021/acschembio.1c00422 | DOI Listing |
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