AI Article Synopsis
- Over-expression of the vasR2 gene in the bacteria Verrucosispora sp. NS0172 activates previously silent gene clusters responsible for producing certain compounds.
- As a result, researchers partially activated a gene cluster that leads to the biosynthesis of pentaketide ansamycins, isolating two tetraketides and one triketide, along with five known compounds.
- The chemical structures of the newly identified compounds were analyzed using advanced nuclear magnetic resonance (NMR) techniques and high-resolution mass spectrometry (HR-ESI-MS).
Article Abstract
Over-expression of the pathway specific positive regulator gene is an effective way to activate silent gene cluster. In the curret study, the SARP family regulatory gene, vasR2, was over-expressed in strain Verrucosispora sp. NS0172 and the cryptic gene cluster responsible for the biosynthesis of pentaketide ansamycin was partially activated. Two tetraketides (1 and 2) and a triketide (3) ansamycins, together with five known compounds (4-8), were isolated and elucidated from strain NS0172OEvasR2. Their NMR data were completely assigned by analysis of their HR-ESI-MS and H, C NMR, HMQC, HMBC and H-H COSY spectra.
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| http://dx.doi.org/10.1016/S1875-5364(21)60053-5 | DOI Listing |
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