AI Article Synopsis
- Researchers successfully extracted 41 g of over 95% pure Balenine (Bal) from 1 kg of opah muscle using hot water, ion-exchange chromatography, and recrystallization.
- The structure of the purified Bal matched an authentic standard through NMR analysis, confirming its identity.
- Bal exhibited superior antioxidant properties and Fe(II) ion-chelating abilities compared to carnosine and anserine, suggesting that opah has potential as a functional food or source of Bal.
Article Abstract
Balenine (Bal) in opah muscle was extracted using hot water and purified by ion-exchange chromatography and recrystallization to provide 41 g of over 95% pure Bal from 1 kg of opah muscle. The structure of purified Bal was identical to that of an authentic Bal standard by NMR analysis. The antioxidant (ORAC and HORAC values) and Fe(II) ion-chelating abilities of purified Bal were examined by comparison with two major imidazole dipeptides, carnosine (Car) and anserine (Ans). Opah-derived Bal showed significantly higher ORAC and HORAC values and Fe(II) ion-chelating ability at 0.3 mM. In silico molecular simulation revealed that Bal and Car formed hydrogen bonds between the hydrogen atom of the imidazole imino group and the carboxyl carbonyl oxygen, whereas Ans did not. The proposed method for extracting and purifying Bal from opah muscle suggests that opah can be utilized as a functional food or Bal resource.
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| http://dx.doi.org/10.1016/j.foodchem.2021.130343 | DOI Listing |
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