AI Article Synopsis
- VAMS and DUS methods have been developed for collecting dried microsamples of low-stability peptide hormones for anti-doping testing.
- The analytical processes were optimized, achieving high sensitivity (0.3-10 ng/mL), precision (RSD% < 6.6 %), and solid extraction yields (78-91 %).
- Dried urine microsamples showed better stability than fluid urine, with lower degradation rates during storage, making them suitable for reliable anti-doping tests.
Article Abstract
Volumetric absorptive microsampling (VAMS) and dried urine spot (DUS) strategies were applied for the collection of dried microsamples for anti-doping testing of low-stability peptide hormones and growth factors prohibited by the World Anti-Doping Agency (WADA). Drying, storage and transport conditions, as well as pretreatment steps, were optimised before liquid chromatography - tandem mass spectrometry (LC-MS/MS) analysis. The analytical method has been fully validated in terms of sensitivity (limits of quantitation 0.3-10 ng/mL), precision (RSD% < 6.6 %) and extraction yields (78-91 %). Dried microsample stability studies (90 days) have been performed and compared to fluid urine stability. Significantly higher losses have been observed in fluid urine stored at -20 °C (up to 55 %) and -80 °C (up to 29 %) than in dried urine microsamples stored at room temperature (< 19 %). The final microsampling and analysis protocols allow the collection of urine microvolumes, unlikely to be tampered, stably storable and shippable with no particular precautions for possible anti-doping testing of prohibited peptides and hormones.
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| http://dx.doi.org/10.1016/j.jpba.2021.114234 | DOI Listing |
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