Dihydro-Si-rhodamine for live-cell localization microscopy.

Chem Commun (Camb)

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China.

Published: July 2021

AI Article Synopsis

  • The study introduces DH-SiR, a new fluorophore that combines both photo-activatable and photo-switchable fluorescence properties.
  • This innovative probe is derived from the reduction of Si-rhodamine, a known dye.
  • The research demonstrates the potential of DH-SiR for enhancing localization microscopy using super-resolution techniques.

Article Abstract

Fluorophores with photo-modulatory fluorescence properties are valuable for cutting-edge localization microscopy. The existing probes are either photo-activatable, or photo-switchable, but not both. We report a probe (DH-SiR), a leuco-dye obtained by reduction of Si-rhodamine, with both photo-activatable and photo-switchable fluorescence. The potential for super-resolution microscopy was showcased.

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Source
http://dx.doi.org/10.1039/d1cc02596aDOI Listing

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