AI Article Synopsis
- * Researchers used advanced sequencing tools to analyze a specific gene from blood samples of HIV patients showing high levels of the parasite.
- * They found diverse strains of the parasite within individual patients, which helps understand the genetic variation of the infection and its effects on disease severity.
Article Abstract
Chagas disease, caused by Trypanosoma cruzi, can reactivate and cause severe acute disease in immunocompromised patients such as those infected with human immunodeficiency virus (HIV). We conducted amplicon deep sequencing of a 327-bp fragment of the tcscd5 gene using an Ion Torrent PGM directly from clinical samples from HIV patients with high parasitemia. We describe the within-host diversity, both characterizing the discrete typing unit of the infections and confirming the presence of multistrain infections, directly from clinical samples. This method can rapidly provide information on the genetic diversity of T. cruzi infection, which can have direct impacts on clinical disease.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764271 | PMC |
| http://dx.doi.org/10.1093/infdis/jiab350 | DOI Listing |
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