Mixed phytochemicals mediated synthesis of copper nanoparticles for anticancer and larvicidal applications.

The synthesis of copper nanoparticles (CuNPs) using () R.Br extract is defined in this article as being convenient, environmentally friendly, and non-toxic. UV-visible spectrophotometry, FT-IR, XRD, particle size analyser, SEM-EDAX and TEM methods were used to describe the physicochemical properties of extract mediated synthesized CuNPs (-CuNPs). The CuNPs synthesized was found to be monodispersed and spherical, with an average size of 15 nm. Gas chromatography and mass spectrometry (GC-MS) research revealed that the R.Br plant extract contains various phytochemical compounds. The properties of -CuNPs were verified by the findings of characterization tests. Via molecular docking experiments with established targets, the underlying mechanisms of cytotoxicity against breast cancer and larvicidal behaviour against of -CuNPs were investigated. Interestingly, cytotoxicity studies showed 50% cell death (IC) of CuNPs treated MCF-7 cells and Vero Cells (Kidney epithelial cells) were displayed at 119.23 μg.mL and 898.75 μg.mL, respectively. Also, CuNPs showed least LC and LC values for larvicidal activity against were of 32.10 μg.mL and 21.70 μg.mL, respectively. Furthermore, -CuNPs is found to be less toxic and biocompatible in haemolytic assays. The findings clearly showed that biosynthesized -CuNPs have been used as a possible anticancer and larvicidal agent, as well as being environmentally friendly.