We focused our study on the 12 recently identified putative odorant carrier proteins in the ectoparasitic mite, Varroa destructor. Here we show, via an exclusion of the chemosensory appendages (forelegs and gnathosoma) that transcripts of five of the 12 genes were significantly lower, suggesting that they are likely involved in carrying host volatiles. Specifically, three transcripts were found to be foreleg-specific while the other two transcripts were expressed in both the forelegs and gnathosoma. We focused on one of the highly expressed and foreleg-specific transcript Vd40090, which encodes a Niemann-Pick disease protein type C2 (NPC2) protein. Effects of dsRNA-mediated silencing of Vd40090 were first measured by quantifying the transcript levels of genes that encode other putative odorant carrier proteins as well as reproduction related proteins. In addition, the impact of silencing on mites behaviour and survival was tested. Silencing of Vd40090 effectively disrupted Varroa host selection, acceptance and feeding and significantly impaired the expression of genes that regulate its reproduction in brood cells, resulting in reduced reproduction and survival.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/imb.12722 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A systematic review suggests extension and redefinition of a food-deception pollination syndrome involving anautogenous flies.
Ann Bot
January 2025
CEFE, University of Montpellier, CNRS, EPHE, IRD, CEDEX 5, 34293 Montpellier, France.
Background And Aims: The currently recognized diversity of pollination strategies requires pollination syndromes to be updated. Described a decade ago, kleptomyiophily is a deceptive pollination system in which plants exploit the nutrient-seeking behavior of females of kleptoparasitic flies (Chloropidae and Milichiidae) by olfactorily mimicking their insect host. Such a pollination system was already hypothesized for pollination by biting midges (Ceratopogonidae) but has never been formalized.
View Article and Find Full Text PDFMolecular, cellular, and developmental organization of the mouse vomeronasal organ at single cell resolution.
Elife
December 2024
Stowers Institute for Medical Research, Kansas City, United States.
We have generated single cell transcriptomic atlases of vomeronasal organs (VNO) from juvenile and adult mice. Combined with spatial molecular imaging, we uncover a distinct, previously unidentified class of cells that express the vomeronasal receptors (VRs) and a population of canonical olfactory sensory neurons in the VNO. High-resolution trajectory and cluster analyses reveal the lineage relationship, spatial distribution of cell types, and a putative cascade of molecular events that specify the V1r, V2r, and OR lineages from a common stem cell population.
View Article and Find Full Text PDFEvolution of queen pheromone receptor tuning in four honeybee species (Hymenoptera, Apidae, Apis).
iScience
December 2024
Evolution, Genomes, Behaviour and Ecology, IDEEV, Université Paris-Saclay, CNRS, IRD, 12 Route 128, 91190 Gif-sur-Yvette, France.
Honeybees (genus: Apis) use a plethora of pheromones for intraspecific communication. The primary compound produced by the queen's mandibular glands, 9-ODA, is involved in mating in all Apis species. It is the ligand of the most highly expressed olfactory receptor in males of : OR11.
View Article and Find Full Text PDFScreening, validation and functional characterization of genes encoding proteins that interact with sensory neuron membrane protein 1b (SNMP1b) from Cyrtotrachelus buqueti (Coleoptera: Curculionidae).
Int J Biol Macromol
January 2025
National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.
Article Synopsis
- Sensory neuron membrane proteins (SNMPs) are crucial for the olfactory system in insects, but studies on their function outside Drosophila, particularly in beetles like Cyrtotrachelus buqueti, are rare.
- In this research, the SNMP1 variant (CbuqSNMP1b) was found to interact with a protein called CbuqOBP1, identified through a yeast two-hybrid system using male C. buqueti antennae as a source for prey protein.
- When CbuqSNMP1b and CbuqOBP1 expression were knocked down, the male beetles showed significantly decreased behavioral responses to specific odors, enhancing our understanding of the role
Involvement of a Microplusin-like Gene (HlonML-1) in the Olfactory Chemosensation of Expression, RNA Silencing, and Behavioral Implications.
Microorganisms
November 2024
Key Laboratory of Animal Parasitology of the Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
The study of tick olfaction is relatively new compared to that of insects, and the molecular mechanisms involved remain poorly understood. Despite several potential chemosensory genes identified in multiple tick species, these are yet to be validated through independent functional experiments. In this research, we cloned and analyzed a microplusin-like gene, HlonML-1, and investigated its role in the chemosensory activities of .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!