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Investigation of the lipidomic changes in differentiated glioblastoma cells after drug treatment using MALDI-MS. | LitMetric

Investigation of the lipidomic changes in differentiated glioblastoma cells after drug treatment using MALDI-MS.

Talanta

Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing, 100084, China. Electronic address:

Published: October 2021

AI Article Synopsis

  • The study emphasizes the importance of lipid differences between tumor and normal tissues for diagnosis and treatment, particularly in glioblastoma, a type of brain tumor known for poor differentiation.
  • Using matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), the research examined changes in lipid profiles of glioblastoma cells treated with sodium phenylbutyrate (SPB) and all-trans retinoic acid (ATRA).
  • Results showed that drug treatment led to reduced cell proliferation, changes in cell cycle, improved cell morphology, and distinctive alterations in membrane lipid profiles, suggesting that lipidomic analysis could help evaluate tumor therapies.

Article Abstract

Lipids differences between tumor and normal tissue have been proved to be of diagnostic and therapeutic significance. The research of lipidomics in tumor is more and more important. Mass spectrometry like matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) can be more convenient and informative for lipids researching in biological and clinical researches. Most of malignant tumors like glioblastoma are characterized by incomplete differentiation, so differentiation therapy has made important progress in tumor treatment. Lipid profiles changes after therapy are worthy investigating. In our study, glioblastoma cell line U87-MG cells were treated by inducers of sodium phenylbutyrate (SPB) and all-trans retinoic acid (ATRA). The changes in lipids on cell membrane were profiled by MALDI-MS. The differentiation degree was assessed by cell proliferation, cell cycle, morphology and protein expression before MALDI-MS analysis. Comparing the inducer treated and untreated U87-MG cells, reduced proliferation rate, blocked cell cycle, benign nucleus morphology and changed expression of protein CD133 and glial fibrillary acidic protein (GFAP), were found after drug treatment. Moreover, the lipids of cell membrane presented distinguished differences in the drug treated cells. Most of the glycerophosphocholines (PC) with an increasing abundance are unsaturated PCs (PC (38:1), 816 m/z; PC (36:1), 788 m/z; PC (31:1), 725 m/z), and those decreasing are saturated PCs (PC (32:0), 734 m/z). These results provide the lipidomic differentiation which may be a significant guidance for evaluating the therapeutic effect of tumor therapy.

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Source
http://dx.doi.org/10.1016/j.talanta.2021.122570DOI Listing

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