AI Article Synopsis
- Soluble stable radicals serve as effective spin probes for electron paramagnetic resonance (EPR) spectroscopy and imaging, both in vitro and in vivo.
- A novel perchlorinated triarylmethyl radical, extensively enriched at its central carbon, is synthesized and characterized, highlighting its unique hyperfine splitting.
- This radical's tumbling rate significantly affects the EPR spectrum, enabling precise measurements of microviscosity in various media through spectral simulation and calibration techniques.
Article Abstract
Soluble stable radicals are used as spin probes and spin labels for in vitro and in vivo electron paramagnetic resonance (EPR) spectroscopy and imaging applications. We report the synthesis and characterization of a perchlorinated triarylmethyl radical enriched 99% at the central carbon, . The anisotropy of the hyperfine splitting with the C ( = 26, = 25, = 199.5 MHz) and the ( = 2.0015, = 2.0015, = 2.0040) are responsible for a strong effect of the radical tumbling rate on the EPR spectrum. The rotational correlation time can be determined by spectral simulation or via the line width or the apparent after calibration, so the spin probe can be used to measure media microviscosity with high sensitivity.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8378891 | PMC |
| http://dx.doi.org/10.1021/acs.jpcb.1c03778 | DOI Listing |
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