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How Bacteria Change after Exposure to Silver Nanoformulations: Analysis of the Genome and Outer Membrane Proteome. | LitMetric

AI Article Synopsis

Article Abstract

Objective: the main purpose of this work was to compare the genetic and phenotypic changes of treated with silver nanoformulations ( BW25113 wt, BW25113 AgR, J53, ATCC 11229 wt, ATCC 11229 var. S2 and ATCC 11229 var. S7). Silver, as the metal with promising antibacterial properties, is currently widely used in medicine and the biomedical industry, in both ionic and nanoparticles forms. Silver nanoformulations are usually considered as one type of antibacterial agent, but their physical and chemical properties determine the way of interactions with the bacterial cell, the mode of action, and the bacterial cell response to silver.

Methods: the changes in the bacterial genome, resulting from the treatment of bacteria with various silver nanoformulations, were verified by analyzing of genes (selected with mutfunc) and their conservative and non-conservative mutations selected with BLOSUM62. The phenotype was verified using an outer membrane proteome analysis (OMP isolation, 2-DE electrophoresis, and MS protein identification).

Results: the variety of genetic and phenotypic changes in strains depends on the type of silver used for bacteria treatment. The most changes were identified in ATCC 11229 treated with silver nanoformulation signed as S2 ( ATCC 11229 var. S2). We pinpointed 39 genes encoding proteins located in the outer membrane, 40 genes of their regulators, and 22 genes related to other outer membrane structures, such as flagellum, fimbria, lipopolysaccharide (LPS), or exopolysaccharide in this strain. Optical density of OmpC protein in electropherograms decreased after exposure to silver nanoformulation S7 (noticed in ATCC 11229 var. S7), and increased after treatment with the other silver nanoformulations (SNF) marked as S2 (noticed in ATCC 11229 var. S2). Increase of FliC protein optical density was identified in turn after Ag+ treatment (noticed in AgR).

Conclusion: the results show that silver nanoformulations (SNF) exerts a selective pressure on bacteria causing both conservative and non-conservative mutations. The proteomic approach revealed that the levels of some proteins have changed after treatment with appropriate SNF.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8308822PMC
http://dx.doi.org/10.3390/pathogens10070817DOI Listing

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