Larvicidal Activities against of Supernatant and Pellet Fractions from Cultured spp. Isolated from Amazonian Microenvironments.

The mosquito is the primary vector of Dengue, Chikungunya and Zika causing major problems for public health, which requires new strategies for its control, like the use of entomopathogenic microorganisms. In this study, bacteria from various Amazonian environments were isolated and tested for their pathogenicity to larvae. Following thermal shock to select sporulated spp., 77 bacterial strains were isolated. Molecular identification per 16S RNA sequences revealed that the assembled strains contained several species of the genus and one species each of , , , and . Among the isolated sp. strains, 19 showed larvicidal activity against . Two strains of also displayed larvicidal activity. For the first time, larvicidal activity against was identified for a strain of . Supernatant and pellet fractions of bacterial cultures were tested separately for larvicidal activities. Eight strains contained isolated fractions resulting in at least 50% mortality when tested at a concentration of 5 mg/mL. Further studies are needed to characterize the active larvicidal metabolites produced by these microorganisms and define their mechanisms of action.