AI Article Synopsis
- Several types of olfactometers, including the Y-tube and high-throughput screening system (HITSS), are used to test mosquito responses to attractants and repellents, with the latter primarily focused on repellents so far.
- The study evaluated the effectiveness of four lactic-acid-based and two non-lactic-acid-based lures on various mosquito species, revealing that specific lures attracted both pyrethroid-susceptible and -resistant mosquitoes but showed different responses based on day or night feeding habits.
- The findings suggest that while HITSS can identify attractive lures, more research is needed to create species-specific attractants and to confirm results in semi-field conditions.
Article Abstract
Several types of olfactometers have been used to evaluate mosquito responses to agents that mimic natural volatiles that repel or attract. The Y-tube olfactometer has been widely used to study repellents and attractants, while the high-throughput screening system assay has only been used to study repellents. Whether the high-throughput screening system assay is suitable for evaluating attractants is unknown. We evaluated the responses to four lactic-acid-based mixtures and two non-lactic-acid-based chemical lure candidates using the high-throughput screening system (HITSS) for three mosquito species (laboratory strains and field populations of both (L.) and Say.; laboratory strain of Theobald) under laboratory-controlled conditions. HITSS assay results showed that KU-lure #1 elicited the greatest percent attraction for pyrethroid-resistant and -susceptible KU-lure #6 elicited the strongest attractive response for pyrethroid-susceptible and -resistant and pyrethroid-susceptible . The response to the lures from each species was independent of the pyrethroid susceptibility status (, = 0.825; , 0.056). However, a significant difference in attraction to KU-lure #6 was observed between diurnal and nocturnal mosquitoes ( vs. , = 0.014; vs. , = 0.001). The laboratory-level HITSS assay effectively selects potential lure candidates. Because the host-seeking behavior differs between mosquito species, further studies are needed to develop species-specific attractants. Additional studies in semi-field screen houses using commercial traps are necessary to evaluate the accuracy of these laboratory assay results.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8226803 | PMC |
| http://dx.doi.org/10.3390/insects12060528 | DOI Listing |
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