Natural resistance-associated macrophage proteins (Nramps) are specific metal transporters in plants with different functions among various species. The evolutionary and functional information of the gene family in has not been previously reported in detail. To identify the genes in , we performed genome-wide identification, characterization, classification, and cis-elements analysis among 22 species with 138 amino acid sequences. We also conducted chromosomal localization and analyzed the synteny relationship, promoter, subcellular localization, and expression patterns in . β-Glucuronidase staining indicated that and mainly accumulated in the root and joint between mother and daughter frond. Moreover, was also widely displayed in the frond. was intensively distributed in the root and frond. Quantitative real-time PCR results proved that the gene expression level was influenced by Cd stress, especially in response to Fe or Mn deficiency. The study provides detailed information on the gene family and their distribution and expression, laying a beneficial foundation for functional research.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232720 | PMC |
http://dx.doi.org/10.3390/ijms22126414 | DOI Listing |
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