AI Article Synopsis
- A specific yeast species lives in the human gut and its ability to transition between two forms (white and opaque) is controlled by a key transcription factor, which affects its metabolism and commensal relationship with the host.* -
- Proteomic analysis shows that when this transcription factor is overexpressed, the yeast struggles to utilize certain sugars (like trehalose and xylose) due to lower levels of isocitrate lyase, linking these metabolic changes to its commensal status.* -
- Mutants lacking proper function of this transcription factor show reduced fitness in the mouse gut, indicating the glyoxylate shunt’s role in helping the yeast adapt as a commensal organism, independent of the transcription factor
Article Abstract
is a commensal yeast that inhabits the gastrointestinal tract of humans. The master regulator of the white-opaque transition has been implicated in the adaptation to this commensal status. A proteomic analysis of cells overexpressing this transcription factor () suggested an altered metabolism of carbon sources and a phenotypic analysis confirmed this alteration. The cells are deficient in using trehalose and xylose and are unable to use 2C sources, which is consistent with a reduction in the amount of Icl1, the isocitrate lyase enzyme. The mutants overexpressing are deficient in the production of phloxine B positive cells, a main characteristic of opaque cells, a phenotype also observed in mating type hemizygous cells, suggesting the involvement of Icl1 in the adaptation to the commensal state. In fact, cells have reduced fitness in mouse gastrointestinal tract as compared with essentially isogenic heterozygous / but overproduction of in an mutant does not restore fitness. These results implicate the glyoxylate shunt in the adaptation to commensalism of by mechanisms that are partially independent of .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8304919 | PMC |
| http://dx.doi.org/10.3390/jof7070502 | DOI Listing |
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