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Rice () TIR1 and 5'adamantyl-IAA Significantly Improve the Auxin-Inducible Degron System in . | LitMetric

Rice () TIR1 and 5'adamantyl-IAA Significantly Improve the Auxin-Inducible Degron System in .

Genes (Basel)

Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton BN1 9RQ, UK.

Published: June 2021

The auxin-inducible degron (AID) system is a powerful tool to induce targeted degradation of proteins in eukaryotic model organisms. The efficiency of the existing AID system is limited due to the fusion of the F-box protein TIR1 protein to the SCF component, Skp1 (Skp1-TIR1). Here, we report an improved AID system for that uses the TIR1 from (OsTIR1) not fused to Skp1. Furthermore, we demonstrate that degradation efficiency can be improved by pairing an OsTIR1 auxin-binding site mutant, OsTIR1, with an auxin analogue, 5'adamantyl-IAA (AID2). We provide evidence for the enhanced functionality of the OsTIR1 AID and AID2 systems by application to the essential DNA replication factor Mcm4 and to a non-essential recombination protein, Rad52. Unlike AID, no detectable auxin-independent depletion of AID-tagged proteins was observed using AID2.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229956PMC
http://dx.doi.org/10.3390/genes12060882DOI Listing

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