Survey on the Presence of Bacterial, Fungal and Helminthic Agents in Off-Leash Dog Parks Located in Urban Areas in Central-Italy.

Off-leash dog parks are designated public spaces where dogs can move freely, under their owners' supervision. These areas, allowing animals to socialize and move freely, are fundamental for dogs' welfare. However, different pathogens, even zoonotic, may be excreted by the attending animals and contaminate the environment. The aim of the present study was to verify the occurrence of bacterial, fungal and parasitic pathogens in off-leash dog parks located in Florence (central Italy). Between March and May 2019, 83 fecal samples, 43 soil samples and 23 water samples (from fountains and puddles) collected from 26 off-leash fenced areas were examined. Fecal samples scored positive for spp. ( = 7), ( = 4),   eggs ( = 2) and eggs ( = 1). Keratinophilic geophilic fungi (mostly ) were recovered from 43 soil samples belonging to 23 out of 26 parks, along with in a single case. spp. was never isolated from water samples, while sp. was cultured in two cases, alone and in association with . These results show that dogs did not act as important carriers for the investigated bacterial and parasitic pathogens, although examined areas may represent a risk for the spreading of some dermatophytoses to both pets and their owners. Periodical examinations to assess the main bacteriological, parasitological and mycological pathogens in different samples collected in off-leash dog parks should be carried out in a One-Health perspective.