Background: The purpose of this study was to explore the role and underlying mechanism of miR-504 and RBM4 in gastric cancer.
Methods: The qRT-PCR or Western blot was performed to determine the expressions of miR-504 and RBM4 in the gastric cancer tissues and normal tissues. Human SGC-7901 cells were transfected with miR-504 mimic/inhibitor or pcDNA-RBM4. Cell proliferation and cell apoptosis were assessed by colony formation assay and flow cytometry, respectively. Luciferase reporter gene assays were used to investigate interactions between miR-504 and RBM4 in SGC-7901 cells.
Results: The relative expression of miR-504 was significantly upregulated in the gastric cancer group ( = 25) than in the paired normal group ( = 25), but the relative RBM4 expression was remarkably downregulated in the gastric tumor group, compared with the normal group. Additionally, miR-504 overexpression increased the viability of gastric cancer cells. Moreover, RBM4 is a functional target of miR-504 in gastric cancer cells. miR-504 was further confirmed to promote SGC-7901 cell proliferation and inhibit cell apoptosis by downregulation RBM4 in vitro.
Conclusions: miR-504 promotes gastric cancer cell proliferation and inhibits cell apoptosis by targeting RBM4, and this provides a potential diagnostic biomarker and treatment for patients with gastric cancer.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203371 | PMC |
| http://dx.doi.org/10.1155/2021/5555950 | DOI Listing |
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