AI Article Synopsis
- Non-small cell lung carcinoma (NSCLC) is a severe form of lung cancer influenced by microRNAs, particularly focusing on the role of miR-770 in cancer progression.
- A bioinformatics tool was utilized to analyze differentially expressed miRNAs, leading to the discovery that miR-770 was downregulated in NSCLC and its agomir could reduce cell proliferation and metastasis by inducing apoptosis.
- The study concluded that exosomal miR-770 inhibits NSCLC invasion by targeting MAP3K1 and reducing M2 macrophage polarization, suggesting a potential new therapeutic approach for NSCLC treatment.
Article Abstract
Background: Non-small cell lung carcinoma (NSCLC) is a type lung cancer with high malignant behaviors. MicroRNAs (miRNAs) are known to be involved in progression of NSCLC. In order to explore potential targets for the treatment of NSCLC, bioinformatics tool was used to analyze differential expressed miRNAs between NSCLC and adjacent normal tissues.
Methods: Bioinformatics tool was used to find potential targets for NSCLC. Cell proliferation was investigated by Ki67 staining. Cell apoptosis was measured by flow cytometry. mRNA and protein expression in NSCLC cells were detected by RT-qPCR and Western-blot, respectively. Transwell assay was performed to test the cell migration and invasion. In order to investigate the function of exosomal miRNA in NSCLC, model of NSCLC was constructed.
Results: MiR-770 was identified to be downregulated in NSCLC, and miR-770 agomir could significantly inhibit NSCLC cell proliferation through inducing the apoptosis. Additionally, the metastasis of NSCLC cells was decreased by miR-770 agomir. MAP3K1 was identified to be the target mRNA of miR-770. Meanwhile, tumor cell-derived exosomal miR-770 inhibited M2 macrophage polarization downregulation of MAP3K1, which in turn suppressed NSCLC cell invasion. Besides, tumor cell-derived exosomal miR-770 markedly decreased NSCLC tumor growth through suppressing M2 macrophage polarization.
Conclusion: Tumor cell-derived exosomal miR-770 inhibits M2 macrophage polarization to inhibit the invasion of NSCLC cells targeting MAP3K1. Thus, this study provided a new strategy for the treatment of NSCLC.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8236888 | PMC |
| http://dx.doi.org/10.3389/fcell.2021.679658 | DOI Listing |
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