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Quorum quenching effects of linoleic and stearic acids on outer membrane vesicle-mediated virulence in .
Biofouling
December 2024
Department of Integrative Biology, School of Biosciences and Technology, Vellore Institute of Technology, Katpadi, Vellore, India.
is a pathogenic bacterium that can infect humans and animals, yet the role of its outer membrane vesicles (OMVs) in mediating pathogenicity remains underexplored. This study evaluated the effects of linoleic acid (LA) and stearic acid (SA) on quorum sensing (QS)-mediated violacein production, biofilm formation, and OMV biogenesis in . Our findings revealed that 2 mM LA and 1 mM SA effectively quench QS, leading to a significant reduction in violacein production, biofilm formation, and OMV biogenesis.
View Article and Find Full Text PDFEvaluation of HPLC Profile, Antioxidant, Quorum Sensing, Biofilm, Swarming Motility, and Enzyme Inhibition Activities of Conventional and Green Extracts of .
Food Sci Nutr
December 2024
Food Quality Control and Analysis Program, Ula Ali Kocman Vocational School Mugla Sitki Kocman University Ula Mugla Turkey.
The current study aims to prepare a green extract using a new method in addition to conventional extraction methods including; methanolic and ultrasonic extraction of , to compare their phenolic composition utilizing high-performance liquid chromatograph equipped with a diode array detector (HPLC-DAD), anti-bacterial, anti-oxidant, and enzyme inhibition activities. The results of HPLC-DAD analysis showed that Rosmarinic acid was found the highest amount in the methanolic extract followed by ultrasonic and green extracts as 169.7 ± 0.
View Article and Find Full Text PDFQuorum sensing inhibition evaluation method: An experiment-based microbiology laboratory course.
Biochem Mol Biol Educ
December 2024
Chemistry Department, Faculty of Sciences, University of Beira Interior, Covilhã, Portugal.
Bacteria have developed a cell-to-cell communication system called quorum sensing (QS), allowing them to regulate group behavior and synchronize the expression of virulence factors, responsible for increasing their infection capacity and resistance to antimicrobials. Although the control of microbial infections through the inhibition of microbial growth has traditionally been the basis of antimicrobial chemotherapy, the emergence of antimicrobial resistance has led to the search for new microbial control strategies, namely through the inhibition of QS. Among the agents studied to inhibit this bacterial communication are essential oils (EO), which are considered very effective QS inhibitors.
View Article and Find Full Text PDFBacterial contamination of mobile handwashing stations in hospital settings in the Democratic Republic of the Congo.
Antimicrob Resist Infect Control
December 2024
Department of Microbiology, Immunology and Transplantation, KU Leuven, Louvain, Belgium.
Background: As part of the containment of the COVID-19 pandemic, mobile handwashing stations (mHWS) were deployed in healthcare facilities in low-resource settings. We assessed mHWS in hospitals in the Democratic Republic of the Congo for contamination with Gram-negative bacteria.
Methods: Water and soap samples of in-use mHWS in hospitals in Kinshasa and Lubumbashi were quantitatively cultured for Gram-negative bacteria which were tested for antibiotic susceptibility.
Rapid progression of post-traumatic endophthalmitis caused by Chromobacterium violaceum, a rare, virulent, ubiquitous pathogen.
J Fr Ophtalmol
December 2024
Department of Medicine, Suddhavej Hospital, Faculty of Medicine, Mahasarakham University, 44000 Maha Sarakham, Thailand. Electronic address:
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