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Global Protein Interactome Mapping in Rice Using Barcode-Indexed PCR Coupled with HiFi Long-Read Sequencing.
Adv Sci (Weinh)
January 2025
State Key Lab of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou, 311400, China.
Establishing the protein-protein interaction network sheds light on functional genomics studies by providing insights from known counterparts. However, the rice interactome has barely been studied due to the lack of massive, reliable, and cost-effective methodologies. Here, the development of a barcode-indexed PCR coupled with HiFi long-read sequencing pipeline (BIP-seq) is reported for high throughput Protein Protein Interaction (PPI)identification.
View Article and Find Full Text PDFThe combination treatment of RC48 and STAT3 inhibitor acts as a promising therapeutic strategy for basal bladder cancer.
Front Immunol
January 2025
Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China.
As an antibody-drug conjugate (ADC), disitamab vedotin (RC48) is a promising treatment targeting ERBB2 for locally advanced and metastatic bladder cancer (BLCA). However, the subtype heterogeneity of muscle-invasive bladder cancer (MIBC) often leads to different therapeutic outcomes. In our study, we aim to explore sensitivity differences and mechanisms of different molecular subtypes of MIBC to RC48 treatment and develop a strategy for combination therapy against cancer.
View Article and Find Full Text PDFCoupling of Li-Fe: Li Isotope Fractionation during Sorption onto Fe-Oxides.
ACS Earth Space Chem
January 2025
Department of Earth Sciences, Utrecht University, 3584 CB Utrecht, The Netherlands.
Chemical weathering processes play a key role in regulating the global climate over geological time scales. Lithium (Li) isotope compositions have proven to be a robust proxy for tracing weathering processes that produce secondary minerals, such as clays and oxides, with a focus often placed on Li adsorption to, or incorporation into, clay minerals. In addition, the interaction between Li and Fe-oxides has long been assumed and discussed based on field observations, but experimental constraints on this process are lacking.
View Article and Find Full Text PDFDouble-Negative CD4 CD8 Absolute Count Plays a Mediating Role in the Causal Relationship Between Plasma Lipids and Parkinson's Disease: A Mendel Randomized Study.
Mol Neurobiol
January 2025
Department of Neurology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Xuhui District, No. 725 South Wan-Ping Road, Shanghai, 200032, China.
According to certain research, there might be a connection between Parkinson's disease and plasma lipidome. However, the causal effects between plasma lipidome and Parkinson's disease and whether immune cells act as a mediator remain unclear. According to some research, plasma lipids are an important risk factor for Parkinson's disease, however, whether there is a causative connection between the two is unclear.
View Article and Find Full Text PDFMechanism of solid electrolyte interphase film formation using ethylene carbonate-based local high concentration electrolyte in sodium-ion batteries.
J Colloid Interface Sci
January 2025
School of Chemistry, South China Normal University, Guangzhou 510006, People's Republic of China. Electronic address:
Sodium-ion batteries (SIBs) have the advantages of abundant resources and low cost, making them potential candidates for the next-generation large-scale energy storage technology. However, the capacity fade during cycling used in sodium-ion batteries is a major challenge. The rational design of the electrolyte is one of the ways to solve these problems.
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