Determination of antioxidant activity and antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of (L.) Pruski.

Background And Aim: (L.) Pruski is a well-known medicinal plant that is widely used in traditional medicine. This study was conducted to evaluate the antioxidant and antineoplastic properties of leaf.

Experimental Procedure: Methanol was used as a solvent to prepare leaf extract from the dried leaf of (MESL). We have evaluated the total phenolic and flavonoid contents of MESL, and using five different assays; we have also evaluated the antioxidant property of MESL. antineoplastic activity of MESL against Ehrlich ascites carcinoma (EAC) cells in experimental mice was also explored.

Results: The phytochemical analysis of MESL exhibited the total phenolic, and total flavonoid contents in the dry extracts were 32.86 mg/gm of gallic acid equivalent and 127.26 mg/gm of catechin equivalent, respectively. Moreover, MESL also showed promising scavenging activity in all assays (IC value ≥70.5 μg/mL). In the antineoplastic assay, MESL showed increased EAC cell death at the doses of 50 mg/kg and 100 mg/kg body weight. MESL administration also induced apoptosis of EAC cells, significantly inhibited EAC cell growth, and increased the life span of EAC cell-bearing mice compared to EAC cell-bearing control mice.

Conclusions: All findings of this study suggest potential antioxidant and antineoplastic properties of MESL.