AI Article Synopsis
- Achyranthis Bidentatae Radix and Semen Vaccariae are traditional Chinese medicines known for treating migraine and erectile dysfunction (ED), and this study aimed to verify their effectiveness in improving migraine-induced ED.
- The research utilized network pharmacology to identify key targets and pathways, establishing a migraine-induced ED model in rats, and evaluated erectile function along with histological and molecular analyses.
- Findings showed that the Myosin light chain kinase-Calcium (MLCK-CaM) signaling pathway is significantly involved, with increased levels of MLCK mRNA and protein in the treated rats, indicating that these herbal medicines may regulate ED via this pathway.
Article Abstract
Background And Aim: Achyranthis Bidentatae Radix plus Semen Vaccariae are traditional Chinese medicines, which have been widely applied in the treatment of migraine and Erectile Dysfunction (ED) for many years. This study verified the effect of Achyranthis Bidentatae Radix plus Semen Vaccariae in improving migraine-induced ED and explored its potential mechanism.
Methods: Key targets and signaling pathways of Achyranthis Bidentatae Radix plus Semen Vaccariae in migraine-induced erectile dysfunction treatment were predicted by network pharmacology. A rat model of migraine was established by nitroglycerin injection. Apomorphine was injected into rats to screen the migraine-induced erectile dysfunction model, Achyranthis Bidentatae Radix-Semen Vaccariae granule suspension administered, and erectile function evaluated. Hematoxylin and eosin staining was used to compare the histological structure of the penile tissue, while RT-qPCR and Western blotting were used to determine mRNA and protein levels, respectively.
Results: Screening allowed us to identify common targets for migraine and ED; the signaling pathway exhibiting the greatest change was the Myosin light chain kinase- Calcium (MLCK-CaM) signal pathway. From Western blotting and RT-qPCR, we found that the levels of MLCK mRNA and protein in rats from Group B rats were significantly higher (P <0.05) than those in Groups A and C. Furthermore, the mRNA and protein levels of CaM were significantly higher in Group B (P <0.05) than in Groups A and C.
Conclusion: Data indicate that the regulatory effects of Achyranthis Bidentatae Radix plus Semen Vaccariae on migraine-induced ED in a rat model are mediated by the MLCK-CaM signaling pathway.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2174/1386207324666210628105233 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Isolation and identification of two novel PDE-5 inhibitors illegally added to pressed candies.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
January 2025
Shanxi Key Laboratory of Food and Drug Safety Prevention and Control, Inspection and Testing Center of Shanxi Province, Taiyuan, Shanxi, China.
Two novel phosphodiesterase 5 (PDE-5) inhibitors were detected in pressed candy using high-performance liquid chromatography (HPLC)-diode array detection. Following extraction with acetonitrile and sonication, the compounds were isolated and purified semi-preparative liquid chromatography. Structural characterisation was achieved through high-resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR) spectroscopy.
View Article and Find Full Text PDFLow-cost male urogenital simulator for penile implant surgery training: a 3D printing approach.
3D Print Med
January 2025
Department of Surgical & Interventional Engineering, School of Biomedical Engineering and Imaging Sciences, King's College London, London, UK.
Background: Penile implant surgery is the standard surgical treatment for end-stage erectile dysfunction. However, the growing complexity of modern high-tech penile prostheses has increased the demand for more practical training opportunities. The most advanced contemporary training methods involve simulation training using cadavers, with costs exceeding $5,000 per cadaver, inclusive of biohazard fees.
View Article and Find Full Text PDFRandomized controlled effect of treadmill walking exercise on liver enzymes, psychological burden, and erectile dysfunction in men with hepatitis C.
Prz Gastroenterol
August 2023
Department of Physical Therapy for Internal Medicine and Geriatrics, Faculty of Physical Therapy, October University for Modern Sciences and Arts, 6th October City, Egypt.
Introduction: Exercise is a simple, safe, and affordable solution that can be easily taught to men with chronic illnesses to improve their psychological burden and sexual health.
Aim: Since the role of exercise in ameliorating the psychological burden associated with erectile dysfunction (ED) in hepatitis C (HC) men was not explored, this research aimed to explore this role.
Material And Methods: This HC study was a randomized-controlled exercise-rehabilitation trial.
The associations among platelet count, mean platelet volume, and erectile dysfunction: an observational and Mendelian randomization study.
Sex Med
December 2024
Department of Urology, Xiangya Hospital, Central South University, Changsha 410000, China.
Background: While previous studies have explored the associations and causalities among platelet count (PC), mean platelet volume (MPV), and erectile dysfunction (ED), further investigations are needed to clarify these relationships using advanced methodologies and analyzing specific populations.
Aim: To investigate the associations and causalities among PC, MPV, and ED using observational study and Mendelian randomization (MR) analysis.
Methods: A total of 114 patients with ED and 158 healthy control participants underwent a fasting blood draw to test for PC and MPV along with a comprehensive laboratory examination.
Association of total testosterone levels with cardiometabolic diseases in men with erectile dysfunction.
Sex Med
December 2024
Visiting staff, Division of Urology, Department of Surgery, Yuan's General Hospital, Kaohsiung City 802793, Taiwan, Republic of China.
Background: Both serum testosterone (T) levels and erectile dysfunction (ED) are associated with systemic diseases in men and ED is the most common presenting symptom of hypogonadism.
Aim: To evaluate the association of serum total testosterone (TT) levels with cardiometabolic diseases in men with ED.
Methods: Serum endogenous TT levels were determined to evaluate their associations with cardiometabolic diseases in men with ED in outpatient clinics.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!