A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: Attempt to read property "Count" on bool

Filename: helpers/my_audit_helper.php

Line Number: 3100

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium. | LitMetric

Role of hepcidin in oxidative stress and cell death of cultured mouse renal collecting duct cells: protection against iron and sensitization to cadmium.

Arch Toxicol

Faculty of Health, Institute of Physiology, Pathophysiology and Toxicology and ZBAF (Centre for Biomedical Education and Research), School of Medicine, Witten/Herdecke University, Stockumer Str 12 (Thyssenhaus), 58453, Witten, Germany.

Published: August 2021

AI Article Synopsis

  • Hepcidin, a liver hormone also found in the kidneys, plays a crucial role in regulating iron levels and protecting against kidney damage caused by iron overload and cadmium toxicity.
  • Research involving kidney cell lines revealed that increasing hepcidin levels can protect against iron-induced cell death while worsening the toxic effects of cadmium, highlighting its complex role in metal ion interactions.
  • Hepcidin appears to bind iron but not cadmium, influencing how these metals affect cellular health and oxidative stress in kidney cells by competing for binding sites in proteins.

Article Abstract

The liver hormone hepcidin regulates systemic iron homeostasis. Hepcidin is also expressed by the kidney, but exclusively in distal nephron segments. Several studies suggest hepcidin protects against kidney damage involving Fe overload. The nephrotoxic non-essential metal ion Cd can displace Fe from cellular biomolecules, causing oxidative stress and cell death. The role of hepcidin in Fe and Cd toxicity was assessed in mouse renal cortical [mCCD(cl.1)] and inner medullary [mIMCD] collecting duct cell lines. Cells were exposed to equipotent Cd (0.5-5 μmol/l) and/or Fe (50-100 μmol/l) for 4-24 h. Hepcidin (Hamp1) was transiently silenced by RNAi or overexpressed by plasmid transfection. Hepcidin or catalase expression were evaluated by RT-PCR, qPCR, immunoblotting or immunofluorescence microscopy, and cell fate by MTT, apoptosis and necrosis assays. Reactive oxygen species (ROS) were detected using CellROX™ Green and catalase activity by fluorometry. Hepcidin upregulation protected against Fe-induced mIMCD cell death by increasing catalase activity and reducing ROS, but exacerbated Cd-induced catalase dysfunction, increasing ROS and cell death. Opposite effects were observed with Hamp1 siRNA. Similar to Hamp1 silencing, increased intracellular Fe prevented Cd damage, ROS formation and catalase disruption whereas chelation of intracellular Fe with desferrioxamine augmented Cd damage, corresponding to hepcidin upregulation. Comparable effects were observed in mCCD(cl.1) cells, indicating equivalent functions of renal hepcidin in different collecting duct segments. In conclusion, hepcidin likely binds Fe, but not Cd. Because Fe and Cd compete for functional binding sites in proteins, hepcidin affects their free metal ion pools and differentially impacts downstream processes and cell fate.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8298330PMC
http://dx.doi.org/10.1007/s00204-021-03106-zDOI Listing

Publication Analysis

Top Keywords

cell death
16
collecting duct
12
hepcidin
11
role hepcidin
8
oxidative stress
8
stress cell
8
mouse renal
8
metal ion
8
cell fate
8
catalase activity
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!