AI Article Synopsis
- Arrays of short, singly-labeled ssDNA oligonucleotides allow for precise detection and capture of specific RNA molecules at the single-molecule level.!
- The article outlines a straightforward enzymatic method that can be performed with basic lab tools to transform PCR oligos into smFISH and RAP probesets.!
- This approach is designed to be quantitative, cost-effective, and adaptable for various research needs.!
Article Abstract
Arrays of short, singly-labeled ssDNA oligonucleotides enable hybridization with single molecule sensitivity and efficient transcript specific RNA capture. Here, we describe a simple, enzymatic protocol that can be carried out using basic laboratory equipment to convert arrays of PCR oligos into smFISH and RAP probesets in a quantitative, cost-efficient and flexible way.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203885 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2750 | DOI Listing |
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