RNA-dependent RNA polymerases (RdRPs) in eukaryotes convert single-stranded RNAs into double-stranded RNAs, thereby amplifying small interfering RNAs that play crucial roles in the regulation of development, maintenance of genome integrity and antiviral immunity. Here, we describe a method of RdRP assay using recombinant RDR6 prepared by an insect expression system. By using this classical biochemical assay, we revealed that RDR6 has a strong template preference for RNAs lacking a poly(A) tail. This simple method will be applicable to other RdRPs in and different organisms.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203873 | PMC |
| http://dx.doi.org/10.21769/BioProtoc.2673 | DOI Listing |
Publication Analysis
Top Keywords
rna-dependent rna
8
rna polymerase
4
polymerase assay
4
assay rdr6
4
rdr6 rna-dependent
4
rna polymerases
4
polymerases rdrps
4
rdrps eukaryotes
4
eukaryotes convert
4
convert single-stranded
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!