Leaf Extract Protects Against Glutamate-Induced Oxidative Toxicity in HT22 Hippocampal Neuronal Cells and Increases Stress Resistance Properties in .

has been used for traditional medicines, food, beverages, and dietary antioxidant supplements. The chemical compositions and biological activities of the fruits and seeds have been extensively investigated. However, the biological effects of the leaves are limited, and its anti-neurodegeneration or antiaging activities are little known. The current work aims to study the beneficial effects of leaf extract on neuroprotective effects in HT22 cells, antiaging, and oxidative stress resistance properties in the model. The ethanol extract was characterized by phytochemical composition using gas/liquid chromatography-mass spectrometry and reversed-phase high-performance liquid chromatography. The beneficial effects of ethanol (VVE) extract on antioxidant properties, neuroprotective effects, and the underlying mechanisms were studied by and studies. In HT22 cells, we found that VVE has a protective effect against glutamate-mediated oxidative stress-induced cell death. The gene expression of cellular antioxidant enzymes such as , and was upregulated by VVE treatment. Moreover, VVE was also shown to alleviate oxidative stress and attenuate reactive oxygen species accumulation in . We demonstrated that VVE could upregulate the expression of stress-response genes and and downregulate the expression of . Our results suggest that the oxidative stress resistance properties of VVE are possibly involved in /FoxO transcription factors. VVE reduced age-related markers (lipofuscin) while did not extend the life span of under normal conditions. This study reports the neuroprotective effect and antioxidant activity of leaf extract and suggests its potential as a dietary or alternative supplement to defend against oxidative stress and age-related diseases.