Sex- and tissue-dependent creatine uptake in response to different creatine monohydrate doses in male and female Sprague-Dawley rats.

Sprague-Dawley rats ( = 32) underwent 8-weeks of creatine monohydrate (CM) supplementation (0, 2.5, 5, and 10 g/L). Total creatine (TCr) concentrations in female white fibre-dominant gastrocnemius (WGAS) and cardiac muscle (HRT) were significantly higher compared with males ( < 0.05). CM supplementation increased TCr concentrations in female WGAS ( < 0.05) and HRT ( < 0.01) and in male red fibre-dominant gastrocnemius muscle (RGAS) ( < 0.05). Future research should further investigate sex-differences in basal levels of TCr and the response to CM supplementation. There is a sex- and tissue-dependant response to CM supplementation in rats.