AI Article Synopsis
- Histones, particularly H2B, are crucial for chromatin structure and assembly, with their protein levels playing a significant role.
- The text outlines protocols for purifying histone H2B from bacteria and differentiating between nucleolar, cytoplasmic, and nucleoplasmic fractions.
- It also discusses methods for studying the ubiquitination and degradation of H2B in these cellular fractions, with additional details available in Liu et al. (2021).
Article Abstract
Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209693 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100601 | DOI Listing |
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