Proteomics Analysis Reveals Bacterial Antibiotics Resistance Mechanism Mediated by Against Enoxacin in .

Bacterial antibiotic resistance is a serious global problem; the underlying regulatory mechanisms are largely elusive. The earlier reports states that the vital role of transcriptional regulators (TRs) in bacterial antibiotic resistance. Therefore, we have investigated the role of TRs on enoxacin (ENX) resistance in in this study. A label-free quantitative proteomics method was utilized to compare the protein profiles of the knockout and wild-type strains under ENX stress. Bioinformatics analysis showed that the deletion of triggers the up-regulated expression of some vital antibiotic resistance proteins in upon ENX stress and thereby reduce the pressure by preventing the activation of SOS repair system. Moreover, directly or indirectly induced at least 11 TRs, which indicates a complicated regulatory network under ENX stress. We also deleted six selected genes in that altered in proteomics data in order to evaluate their roles in ENX stress. Our results showed that genes such as , , , , and are regulated by and may be involved in ENX resistance. Overall, our data demonstrated the important role of in ENX resistance and provided novel insights into the effects of transcriptional regulation on antibiotic resistance in bacteria.