AI Article Synopsis
- * Creating animal models that can selectively silence or overexpress lncRNAs is essential for studying their functions in specific tissues or across whole organisms.
- * CRISPR/Cas technology is a powerful tool that simplifies the manipulation of mouse genomes, making it possible to investigate lncRNA functions in living organisms.
Article Abstract
Long noncoding RNAs (lncRNAs) are implicated in several biological processes and it has been observed that their expression is altered in several diseases. The generation of animal models where selective silencing or overexpression of lncRNAs can be attained is crucial for their biological characterization, since it offers the opportunity to analyze their function at the tissue specific or organismal level. CRISPR/Cas technology is a newly developed tool that allows to easily manipulate the mouse genome, in turn allowing to discover lncRNAs functions in an in vivo context. Here, we provide an overview of how CRISPR/Cas technology can be used to generate transgenic mouse models in which lncRNAs can be studied.
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| http://dx.doi.org/10.1007/978-1-0716-1581-2_14 | DOI Listing |
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