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Insensitivity of dental pulp stem cells migration to substrate stiffness. | LitMetric

Insensitivity of dental pulp stem cells migration to substrate stiffness.

Biomaterials

Inserm UMR-S1121, Centre de Recherche en Biomédecine de Strasbourg (CRBS), 1 rue Eugène Boeckel, 67084, Strasbourg, France; Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, 67000, Strasbourg, France; Fédération de Médecine Translationnelle, Strasbourg, France. Electronic address:

Published: August 2021

AI Article Synopsis

  • Dental pulp stem cells (DPSCs) show potential for regenerating dental pulp, but how the stiffness of their surrounding environment affects their migration remains unclear.* -
  • The study found that DPSCs migrate faster on softer substrates (lower stiffness) and that their motility isn’t significantly affected by the rigidity of the surface, even in mixed rigidity conditions.* -
  • Inhibition of specific cellular components like the Arp2/3 complex reduces migration speed, while the chromatin structure remains stable over time, suggesting that when designing scaffolds for dental pulp regeneration, varying rigidity can be considered without affecting DPSC movement.*

Article Abstract

Dental pulp stem cells (DPSCs) are a promising cell source for regeneration of dental pulp. Migration is a key event but influence of the microenvironment rigidity (5 kPa at the center of dental pulp to 20 GPa for the dentin) is largely unknown. Mechanical signals are transmitted from the extracellular matrix to the cytoskeleton, to the nuclei, and to the chromatin, potentially regulating gene expression. To identify the microenvironmental influence on migration, we analyzed motility on PDMS substrates with stiffness increasing from 1.5 kPa up to 2.5 MPa. We found that migration speed slightly increases as substrate stiffness decreases in correlation with decreasing focal adhesion size. Motility is relatively insensitive to substrate stiffness, even on a bi-rigidity PDMS substrate where DPSCs migrate without preferential direction. Migration is independent of both myosin II activity and YAP translocation after myosin II inhibition. Additionally, inhibition of Arp2/3 complex leads to significant speed decrease for all rigidities, suggesting contribution of the lamellipodia in the migration. Interestingly, the chromatin architecture remains stable after a 7-days exposure on the PDMS substrates for all rigidity. To design scaffold mimicking dental pulp environment, similar DPSCs migration for all rigidity, leaves field open to choose this mechanical parameter.

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Source
http://dx.doi.org/10.1016/j.biomaterials.2021.120969DOI Listing

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