Removal of two cytostatic drugs: bleomycin and vincristine by white-rot fungi - a sorption study.

Purpose: Cytostatic drugs cannot be easily removed by conventional sewage treatment plants, resulting in their ultimate release into aquatic systems where they become a threat. Thus, new technologies which can be used to eliminate these drugs more effectively before they enter the environment are increasingly important. Fungal treatment of wastewaters is a promising and environmentally friendly technology for pharmaceutical remediation. The aim of this work is to examine the biosorption of two cytostatics, bleomycin and vincristine, in the aqueous solution by fungal biomass.

Methods: Five white-rot fungi were used in this study: (CB13), (CB15), (CB14), (BWPH), and (CB8). Tests were conducted on different types of biomass (alive and dead - autoclaved) and in various physico-chemical conditions: varied drug concentrations (5, 10 and 15 mg/L), temperatures (from 15.4 to 29.6 °C), and pH (from 3.2 to 8.8).

Results: The results showed that among alive biomass, (CB8) had the greatest sorption ability for bleomycin and (CB14) worked best for vincristine. The tested sorption process could be described by a pseudo-second order kinetics model. Sorption equilibrium studies demonstrated that for bleomycin Redlich-Peterson, while for vincristine Langmuir model fitted best. The thermodynamic studies showed that the sorption process was endothermic chemisorption for bleomycin, and exothermic physisorption for vincristine. For both drugs the sorption ability increased with an increase of the pH value.

Conclusion: The biosorption on fungal biomass is a favorable alternative to conventional wastewater treatment processes for anticancer drug removal.